EMPIAR-11052
Cryo-EM structure of Bacillus subtilis RNA Polymerase in complex with HelD [3656 multi-frame micrographs composed of 60 frames each in MRC format]
Publication:

Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD

Newing T, Oakley AJ, Miller M, Dawson CJ, Brown SHJ, Bouwer JC, Tolun G, Lewis PJ

Nature communications 11 (2020) 6420-6431

PMID: 33339820

Related EMDB entry:
Deposited:
2020-09-16
Released:
2022-06-07
Last modified:
2022-06-07
Imageset size:
666.21 GB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   3,050,261,124 kilobytes
Compressed size:   698,513,822 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs for Bacillus subtilis RNA Polymerase in complex with HelD
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3656
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.84, 0.84)
Details:
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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)