EMPIAR-11027 - Cryo electron microscopy of in vitro recombinant SAA1.1 amyloid fibrils [multiple data sets in TIFF and JPEG formats]

EMPIAR-11027

Cryo electron microscopy of in vitro recombinant SAA1.1 amyloid fibrils [multiple data sets in TIFF and JPEG formats]

Publication:

AA amyloid fibrils from diseased tissue are structurally different from in vitro formed SAA fibrils

Bansal A, Schmidt M, Rennegarbe M, Haupt C, Liberta F, Stecher S, Puscalau-Girtu I, Biedermann A, Fändrich M

Nature communications 12 (2021)

PMID: 33579941

DOI: 10.1038/s41467-021-21129-z

Related EMDB entry:

EMD-11162 EMD-11163

Deposited:

2021-06-22

Released:

2022-04-25

Last modified:

2022-04-25

Imageset size:

525.41 GB

Imageset DOI:

10.6019/EMPIAR-11027

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multiframe micrographs of ex-vivo murine SAA1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3001

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.04, 1.04)

Details:

Data collection with a Titan Krios EM (300 kV) using a K2 Summit camera, 20 eV slit. Defocus range -0.5 to -2.5, exposure time 12 seconds, dose rate 3.33 e/A^2/sec, total dose 40 e/A^2.
In addition, thumbnails with powerspectra and the gain reference (data/gain_reference) can be found within this entry.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Thumbnails for micrographs of ex-vivo murine SAA1

Category:

micrographs - single frame

Image format:

JPEG

No. of images or tilt series:

3001

Image size:

(1017, 1000)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(3.93, 3.93)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2020-07-15]

PDBj는 2020 년 7 월에 20 주년을 맞이했습니다.

[2019-02-20]

[wwPDB] 고분자결정구조 등록 시 PDBx/mmCIF 형식 파일만 허용됩니다.

[2017-11-16]

11 월 24 일 ~ 26 일, 텔레콤 센터 빌딩 (도쿄 오다이바)에서 개최되는 「사이언스 아고라 2017」에 출전합니다

[2016-01-28]

News on 2016-1-11

See all news