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EMPIAR-10998
In situ single particle classification reveals distinct 60S maturation intermediates in cells [multiple data sets in MRC format]
Publication:

In situ single particle classification reveals distinct 60S maturation intermediates in cells

Lucas BA, Zhang K, Loerch S, Grigorieff N

eLife (2022)

Related EMDB entry:
Deposited:
2022-04-04
Released:
2022-05-03
Last modified:
2022-05-03
Imageset size:
10.47 GB
Imageset DOI:
10.6019/EMPIAR-10998
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • reconstructed volumes
Image Sets
1. Micrographs of untilted lamellae from cryo-FIB milled S. cerevisiae cells
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
28
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.06, 1.06)
Details:
Micrographs were recorded as movies on a Titan Krios at 300 keV equipped with a K3 direct detector and an energy filter, with an exposure rate of 1 e-/Å2 and a total exposure of 30 e-/Å2.
Files:
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2. Templates of S. cerevisiae 60S ribosomal subunits at different stages of maturation, used for 2D template matching
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
3
Image size:
(384, 384)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.06, 1.06)
Details:
3D density maps used as templates in 2D template matching to detect 60S ribosomal subunits in the 2D images of lamellae of S. cerevisiae cells. The density maps were generated with the cryo-EM simulator implemented in cisTEM.
Files:
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3. Scaled maximum intensity projections of SNR values obtained by 2D template matching with the mature 60S template
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
28
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.06, 1.06)
Details:
Maximum intensity projections (MIPs) of the observed SNR values, scaled by subtracting the average SNR, and dividing by the standard deviation of the SNR per pixel. The MIPs display peaks in places where ribosomal subunits were detected by 2D template matching. Peaks are associated with specific x,y locations, defocus and orientations of the targets that correspond with the matching template.
Files:
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4. Scaled maximum intensity projections of SNR values obtained by 2D template matching with the LN60S template
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
28
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.06, 1.06)
Details:
Maximum intensity projections (MIPs) of the observed SNR values, scaled by subtracting the average SNR, and dividing by the standard deviation of the SNR per pixel. The MIPs display peaks in places where ribosomal subunits were detected by 2D template matching. Peaks are associated with specific x,y locations, defocus and orientations of the targets that correspond with the matching template.
Files:
Loading...
5. Scaled maximum intensity projections of SNR values obtained by 2D template matching with the EN60S template
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
28
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.06, 1.06)
Details:
Maximum intensity projections (MIPs) of the observed SNR values, scaled by subtracting the average SNR, and dividing by the standard deviation of the SNR per pixel. The MIPs display peaks in places where ribosomal subunits were detected by 2D template matching. Peaks are associated with specific x,y locations, defocus and orientations of the targets that correspond with the matching template.
Files:
Loading...
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