EMPIAR-10929 - CryoET of presequence protease single particle [multiple data sets in TIFF and MRC formats]

EMPIAR-10929

CryoET of presequence protease single particle [multiple data sets in TIFF and MRC formats]

Publication:

Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition

Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ

Nature communications 13 (2022)

PMID: 35383169

DOI: 10.1038/s41467-022-29322-4

Related EMDB entry:

EMD-25921

Deposited:

2022-01-14

Released:

2022-01-28

Last modified:

2022-10-17

Imageset size:

25.43 GB

Imageset DOI:

10.6019/EMPIAR-10929

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
reconstructed volumes

Image Sets

1. Unaligned K2 tilt image frame stacks

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.331, 1.331)

Details:

Unaligned K2 tif tilt image frame stacks. Frame number increases from 8 at 0 degrees to 13 at high tilts to account for increasing thickness; dose increases from 3.66 to 5.94 e/A^2.
Gain references are in the references/ folder.

Files:

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Binned by 4 tomograms from all 7 tilt-series

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(1200, 1130)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(5.324, 5.324)

Details:

Dose-compensated Tomo3D SIRT binned by 4 reconstructions from Appion-Protomo tilt-series alignments for each tilt-series in the provided frames directory.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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