EMPIAR-10909 - Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and gamma2 subunits, in presence of GABA and nanobody Nb25 [14346 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-10909

Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and gamma2 subunits, in presence of GABA and nanobody Nb25 [14346 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Differential assembly diversifies GABA_A receptor structures and signalling

Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR

Nature (2022)

PMID: 35355020

DOI: 10.1038/s41586-022-04517-3

Related EMDB entry:

EMD-14073 EMD-14072

Deposited:

2021-12-29

Released:

2022-04-04

Last modified:

2022-04-04

Imageset size:

4.21 TB

Imageset DOI:

10.6019/EMPIAR-10909

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of GABA(A) receptors purified from cells expressing alpha4, beta3 and gamma2 subunits

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14346

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.326, 0.326)

Details:

K3 super-resolution movies with a total dose of 47.15 e/A2 and exposure time of 1.31 s.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

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Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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In situ structure determination of conformationally flexible targets using nextPYP.

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CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

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