EMPIAR-10839 - Reconstructed cryo soft X-ray tomography dataset of a treated NIH-3T3 cell with corresponding correlated cryo-3D-SIM channels [300 reconstructed volumes in TIFF format]

EMPIAR-10839

Reconstructed cryo soft X-ray tomography dataset of a treated NIH-3T3 cell with corresponding correlated cryo-3D-SIM channels [300 reconstructed volumes in TIFF format]

Publication:

Correlative 3D cryo X-ray imaging reveals intracellular location and effect of designed antifibrotic protein-nanomaterial hybrids

Groen J, Palanca A, Aires A, Conesa JJ, Maestro D, Rehbein S, Harkiolaki M, Villar AV, Cortajarena AL, Pereiro E

Chemical science 12 (2021) 15090-15103

PMID: 34909150

DOI: 10.1039/d1sc04183e

Related EMDB entry:

Deposited:

2021-10-29

Released:

2021-11-26

Last modified:

2022-01-07

Imageset size:

10.08 GB

Imageset DOI:

10.6019/EMPIAR-10839

Experimental metadata:

Download xml json

Contains:

reconstructed volumes

Image Sets

1. Stitched and correlated 3D reconstruction of a NIH-3T3 cell

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

300

Image size:

(1890, 1590)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

Volume contains 3 channel, 2 of which show the fluorescent data that has been correlated to a SXT stitched tomogaphic reconstruction dataset (also available in the associated Biostudies repository). Voxelsize: 20 x 20 x 10 nm

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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