EMPIAR-10792
Structural mechanism of GTPase-powered ribosome-tRNA movement [multiple data sets in MRC and MRCS formats]
Publication:

Structural mechanism of GTPase-powered ribosome-tRNA movement

Petrychenko V, Peng BZ, de A P Schwarzer AC, Peske F, Rodnina MV, Fischer N

Nature communications 12 (2021)

PMID: 34635670

Deposited:
2021-08-23
Released:
2021-11-10
Last modified:
2021-11-10
Imageset size:
1.06 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • picked particles - single frame - processed
1. Motion-corrected, dose-weighted micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
9300
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Note: Micrographs were obtained from movie-stacks of 20 frames à 1.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "corrected_micrographs.star"
Files:
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2. Shiny particles of non-rotated ribosome particles (C state), obtained by Bayesian polishing in Relion 3.1
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1176423
Image size:
(288, 288)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Shiny particles .star-file & 3D mask for final 3D refinement of C state (EMD-13458): "shiny_Cstate.star" & "mask_Cstate.mrc"
Files:
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3. Shiny particles of rotated ribosome particles, obtained by Bayesian polishing in Relion 3.1
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
103222
Image size:
(288, 288)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Star-files of shiny particles & 3D masks used for final 3D refinements:
- H1 (EMD-13459): "shiny_H1.star" & "mask_H1.mrc"
- H2 (EMD-13460): "shiny_H2.star" & "mask_H2.mrc"
- H1-EF-G-GDP-Pi (EMD-13461): "shiny_H1-EF-G-GDP-Pi.star" & "mask_H1-EF-G-GDP-Pi.mrc"
- H2-EF-G-GDP-Pi (EMD-13462): "shiny_H2-EF-G-GDP-Pi.star" & "mask_H2-EF-G-GDP-Pi.mrc"
- H1-EF-G-GDP (EMD-13463): "shiny_H1-EF-G-GDP.star" & "mask_H1-EF-G-GDP.mrc"
- CHI1-EF-G-GDP (EMD-13464): "shiny_CHI1-EF-G-GDP.star" & "mask_CHI1-EF-G-GDP.mrc"
- CHI2-EF-G-GDP (EMD-13465): "shiny_CHI2-EF-G-GDP.star" & "mask_CHI2-EF-G-GDP.mrc"
Star-files of shiny particles for substates 1-3 of EF-G domain 4 in H1-EF-G-GDP-Pi state (EMD-13461):
- "shiny_H1-EF-G-GDP-Pi_D4_substate1.star"
- "shiny_H1-EF-G-GDP-Pi_D4_substate2.star"
- "shiny_H1-EF-G-GDP-Pi_D4_substate3.star"
Files:
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