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Electron Microscopy Public Image Archive

EMPIAR-10725
Single particle cryo EM dataset of SARS-CoV-2 Spike protein with D614G substitution [10051 multi-frame micrographs composed of 32 frames each in MRC format]
Publication:

The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2

Benton DJ, Wrobel AG, Roustan C, Borg A, Xu P, Martin SR, Rosenthal PB, Skehel JJ, Gamblin SJ

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 33579792

Related EMDB entry:
EMD-12231 EMD-12230 EMD-12229
Deposited:
2021-05-18
Released:
2021-06-11
Last modified:
2021-06-11
Imageset size:
4.51 TB
Imageset DOI:
10.6019/EMPIAR-10725
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   10,539,247,427 kilobytes
Compressed size:  4,839,062,181 kilobytes
Contains:
  • micrographs - multiframe
Image Sets
1. Unaligned multi-frame micrographs of SARS-CoV-2 Spike with D614G substitution
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
10051
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.85, 0.85)
Details:
Uncorrected movies of D614G SARS-CoV-2 spike used to calculate closed, 1 erect RBD and 2 erect RBD forms of this mutant spike. Images were taken on FEI Krios G2 equipped with a Falcon 3 operating in electron counting mode. Images were 40s exposures fractionated into 32 frame gain corrected mrc files, with an accumulated dose of 36.8 e/Å^2. Images were collected with nominal defocus of 1 - 3 µm applied.
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