EMPIAR-10663 - Metavinculin ABD-F-actin complex [1818 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10663

Metavinculin ABD-F-actin complex [1818 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Molecular mechanism for direct actin force-sensing by α-catenin

Mei L, Espinosa de Los Reyes S, Reynolds MJ, Leicher R, Liu S, Alushin GM

eLife 9 (2020)

PMID: 32969337

DOI: 10.7554/elife.62514

Related EMDB entry:

EMD-20844

Deposited:

2021-03-01

Released:

2021-04-06

Last modified:

2021-04-06

Imageset size:

636.70 GB

Imageset DOI:

10.6019/EMPIAR-10663

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned multi-frame micrographs of metavinculin ABD-actin complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1818

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.515, 0.515)

Details:

Unaligned and non-gain corrected, 40-frame movies (K2 counting mode) were collected with using a Titan Krios operating at 300 kV (Rockefeller Krios I). A micrograph accumulates 6 electron per pixel per second with a defocus range of -3.5 to -1.5 um. Micrographs were auto-picked in relion. Gain reference is data/reference_fliprot90.mrc

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