EMPIAR-10621 - Lysosomes as Fiducials for SXT and SIM Correlation [166 tilt series in TIFF format]

EMPIAR-10621

Lysosomes as Fiducials for SXT and SIM Correlation [166 tilt series in TIFF format]

Publication:

Sample preparation strategies for efficient correlation of 3D imaging data at cryogenic temperatures: the journey towards multimodal bioimaging

Okolo CA, Kounatidis I, Groen J, Nahas KL, Balint S, Fish T, Koronfel MA, López-Cortajarena A, Dobbie I, Pereiro E, Harkiolaki M

Nature Protocols (2021)

Related EMDB entry:

Deposited:

2021-01-18

Released:

2021-09-01

Last modified:

2021-09-01

Imageset size:

162.41 MB

Imageset DOI:

10.6019/EMPIAR-10621

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. SXT using Lysosome and non-fluorescent Au as fiducials

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

166

Image size:

(946, 806)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(160.0, 160.0)

Details:

---

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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