EMPIAR-10620 - Lipid Droplets as Fiducials for SXT and SIM Correlation [700 tilt series in MRC format]

EMPIAR-10620

Lipid Droplets as Fiducials for SXT and SIM Correlation [700 tilt series in MRC format]

Publication:

Sample preparation strategies for efficient correlation of 3D imaging data at cryogenic temperatures: the journey towards multimodal bioimaging

Okolo CA, Kounatidis I, Groen J, Nahas KL, Balint S, Fish T, Koronfel MA, López-Cortajarena A, Dobbie I, Pereiro E, Harkiolaki M

Nature Protocols (2021)

Related EMDB entry:

Deposited:

2021-01-18

Released:

2021-03-05

Last modified:

2021-08-20

Imageset size:

5.14 GB

Imageset DOI:

10.6019/EMPIAR-10620

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. SXT using lipid droplets as fiducials

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

700

Image size:

(946, 946)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(100.0, 100.0)

Details:

---

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

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