EMPIAR-10605 - Single particle cryo-EM dataset of Mus musculus mitochondrial complex I with ND6 P25L mutation [1492 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-10605

Single particle cryo-EM dataset of Mus musculus mitochondrial complex I with ND6 P25L mutation [1492 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

Structural basis for a complex I mutation that blocks pathological ROS production

Yin Z, Burger N, Kula-Alwar D, Aksentijević D, Bridges HR, Prag H, Grba D, Viscomi C, James AJ, Mottahedin A, Kreig T, Murphy MP, Hirst J

Nature communications 12 (2021)

PMID: 33514727

DOI: 10.1038/s41467-021-20942-w

Related EMDB entry:

EMD-11811

Deposited:

2020-12-10

Released:

2021-02-17

Last modified:

2021-02-17

Imageset size:

3.40 TB

Imageset DOI:

10.6019/EMPIAR-10605

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Single particle cryo-EM dataset of Mus musculus mitochondrial complex I with ND6 P25L mutation

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1492

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.054, 1.054)

Details:

Micrographs were imaged using a Gatan K2 summit camera in counting mode and GIF Quantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 10s exposures captured in 40 frames and a total dose of 50 electrons Å-2. Double-exposed micrographs were not included in the deposited dataset.

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