EMPIAR-10582 - Single-particle Cryo-EM of the Thermococcus gammatolerans McrB AAA+ hexamer [1605 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10582

Single-particle Cryo-EM of the Thermococcus gammatolerans McrB AAA+ hexamer [1605 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural asymmetry governs the assembly and GTPase activity of McrBC restriction complexes

Niu Y, Suzuki H, Hosford CJ, Walz T, Chappie JS

Nature communications 11 (2020)

PMID: 33219217

DOI: 10.1038/s41467-020-19735-4

Related EMDB entry:

EMD-20865

Deposited:

2020-11-21

Released:

2020-12-04

Last modified:

2020-12-04

Imageset size:

644.61 GB

Imageset DOI:

10.6019/EMPIAR-10582

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the Thermococcus gammatolerans McrB AAA+ hexamer

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1605

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.5, 0.5)

Details:

Gain reference is included as a .mrc file in the same folder with the movie files. The gain reference must be rotated 90 degrees and flipped upside down. Dose per frame: 2 e/A^2

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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