EMPIAR-10500 - High resolution structure of GABAAR beta3 homopentamer [multiple data sets in EER and TIFF formats]

EMPIAR-10500

High resolution structure of GABAAR beta3 homopentamer [multiple data sets in EER and TIFF formats]

Publication:

Single-particle cryo-EM at atomic resolution

Nakane T, Kotecha A, Sente A, McMullan G, Masiulis S, Brown PMGE, Grigoras IT, Malinauskaite L, Malinauskas T, Miehling J, Uchański T, Yu L, Karia D, Pechnikova EV, de Jong E, Keizer J, Bischoff M, McCormack J, Tiemeijer P, Hardwick SW, Chirgadze DY, Murshudov G, Aricescu AR, Scheres SHW

Nature (2020)

PMID: 33087931

DOI: 10.1038/s41586-020-2829-0

Related EMDB entry:

EMD-11657

Deposited:

2020-05-24

Released:

2020-11-13

Last modified:

2020-11-13

Imageset size:

13.05 TB

Imageset DOI:

10.6019/EMPIAR-10500

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. CFEG, Falcon4, with 5 eV filter

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

5573

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.727, 0.727)

Details:

Total dose: 40 e/A2 on sample

Please use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. CFEG, Falcon4, with 5 eV filter, without objective aperture

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

3160

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.727, 0.727)

Details:

Same cautions as "CFEG, Falcon4, with 5 eV filter" apply.

Please use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. XFEG, Falcon3, without filter

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1501

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.827, 0.827)

Details:

The pixel size in the image header (0.8583 A/px) is wrong. The processing was started with
a nominal pixel size of 0.83 A/px, which was later calibrated to 0.827 A/px.

Dose rate: 0.93 e/A2/frame

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. XFEG, Falcon4, with filter but retracted slit

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1244

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.735, 0.735)

Details:

The pixel size in the header (1.00 A/px) is wrong. Please ignore it.

Dose rate: 0.44 e/A2/frame

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. XFEG, Falcon4, with 3 eV filter

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1952

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.735, 0.735)

Details:

Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. XFEG, Falcon4, with 5 eV filter

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1556

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.735, 0.735)

Details:

Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

7. XFEG, Falcon4, with 3 eV filter

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1268

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.735, 0.735)

Details:

Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

8. XFEG, K3, without filter

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1508

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.32741, 0.32741)

Details:

The pixel size in the image header is wrong. The processing was started with
a nominal (super-resolution) pixel size of 0.325 A/px, which was later calibrated to 0.32741 A/px. Please use the provided gain reference (gain.mrc) without any transformation.

Dose rate: 0.96 e/A2/frame

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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