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EMPIAR-10493
Subtomogram averaging and classification of SARS-CoV-2 Spike Proteins on intact virions [multiple data sets in TIFF and MRC formats]
Publication:

Structures and distributions of SARS-CoV-2 spike proteins on intact virions.

Ke Z, Oton J, Qu K, Cortese M, Zila V, McKeane L, Nakane T, Zivanov J, Neufeldt CJ, Cerikan B, Lu JM, Peukes J, Xiong X, Kräusslich HG, Scheres SHW, Bartenschlager R, Briggs JAG

Nature (2020)

PMID: 32805734

Related EMDB entry:
EMD-11494 EMD-11493 EMD-11496 EMD-11495
Deposited:
2020-08-05
Released:
2020-08-19
Last modified:
2020-08-19
Imageset size:
372.39 GB
Imageset DOI:
10.6019/EMPIAR-10493
Experimental metadata:
Download xml json
Contains:
  • tilt series
Image Sets
1. Unaligned Frames from the microscope. Data were recorded
Category:
tilt series
Image format:
TIFF
No. of images or tilt series:
156
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.532, 1.532)
Details:
156 tilt series of unconcentrated SARS-CoV-2 virions with the raw frames. The Frames collected from Titan Krios operated at 300 kV using a Gatan K2 camera. The energy filter used was 20 eV. Data collection was done in SerialEM 3.8.0 using the dose-symmetric scheme (Tilt Series Controler), 3 degrees per tilt step, +/- 60 degrees. 10 Frames were saved per each tilt image. The exposure dose per tilt image is 3.0 electrons per angstrom squared. See mdoc files for the order of the images acquired.

Please use data/gainRef/CountRef_TS_001_000_-0.0.dm4 as the gain reference.
Please see data/mdoc folder for the metadata of the 156 tilt series of unconcentrated SARS-CoV-2 virions
Files:
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2. Aligned and order sorted stack of the tilt series. This is not dose-filtered stack. Associated mdoc files are included in this folder.
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
156
Image size:
(3710, 3838)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.532, 1.532)
Details:
156 tilt series of unconcentrated SARS-CoV-2 virions with aligned stacks and associated mdoc files. The stacks are sorted and not dose-filtered. Data collection was done in SerialEM 3.8.0 using the dose-symmetric scheme (Tilt Series Controler), 3 degrees per tilt step, +/- 60 degrees. The exposure dose per tilt image is 3.0 electrons per angstrom squared. Motion correction was done using alignframes command in IMOD 4.10.30 and the raw frames are in the data/Frames folder.
Files:
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