EMPIAR-10405 - Human apo ferritin frozen on TEM grid with amorphous carbon supporting film [743 multi-frame micrographs composed of 32 frames each in TIFF format]

EMPIAR-10405

Human apo ferritin frozen on TEM grid with amorphous carbon supporting film [743 multi-frame micrographs composed of 32 frames each in TIFF format]

Publication:

Amorphous nickel titanium alloy film: A new choice for cryo electron microscopy sample preparation

Huang X, Zhang L, Wen Z, Chen H, Li S, Ji G, Yin CC, Sun F

Progress in biophysics and molecular biology 156 (2020) 3-13

PMID: 32758492

DOI: 10.1016/j.pbiomolbio.2020.07.009

Related EMDB entry:

EMD-30083

Deposited:

2020-03-26

Released:

2021-08-13

Last modified:

2021-08-13

Imageset size:

226.49 GB

Imageset DOI:

10.6019/EMPIAR-10405

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Human apo ferritin frozen on TEM grid with amorphous carbon supporting film

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

743

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.435, 0.435)

Details:

The raw data were taken in K2 super resolution mode. There are 743 multi-frame stacks without gain correction, motion correction and distortion correction in TIFF format with LZW compression of 8bit. The gain file were also provided in data/C/gain.mrc (7676*7420, 32bit). The distortion parameter were distotaion angle of 26.1, major scale of 1.019 and minor scale of 1. The total dose was 60 e/A2.
Particle positions in corresponding coordinate start file were used to extract particles from bin2 micrographs for final reconstruction.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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