EMPIAR-10358 - CryoEM dataset of sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain of case 2 [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-10358

CryoEM dataset of sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain of case 2 [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Structures of α-synuclein filaments from multiple system atrophy

Schweighauser M, Shi Y, Tarutani A, Kametani F, Murzin AG, Ghetti B, Matsubara T, Tomita T, Ando T, Hasegawa K, Murayama S, Yoshida M, Hasegawa M, Scheres SHW, Goedert M

Nature 585 (2020) 464-469

PMID: 32461689

DOI: 10.1038/s41586-020-2317-6

Related EMDB entry:

EMD-10651 EMD-10652

Deposited:

2020-02-07

Released:

2020-11-18

Last modified:

2020-12-09

Imageset size:

1.11 TB

Imageset DOI:

10.6019/EMPIAR-10358

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5172

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.15, 1.15)

Details:

Movies were recorded automatically using EPU on a K2 camera in counting mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 10.8 second movies contain 48 frames and the pixel size is 1.15 Å/px. The total dose per movie is ~47.5 e/Å^2. The gain reference image is included.

Files:

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2. Dose-weighted aligned micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

5172

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.15, 1.15)

Details:

Movie frames were corrected for beam-induced motion and dose-weighted using RELION’s motion-correction implementation with following arguments:
--first_frame_sum 1 --last_frame_sum -1 --use_own --j 5 --bin_factor 1 --bfactor 150 --angpix 1.15 --voltage 300 --dose_per_frame 0.81 --preexposure 0 --patch_x 4 --patch_y 4 --gainref gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Polished particle stacks

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

3030

Image size:

(220, 220)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.15, 1.15)

Details:

Bayesian polishing was performed in RELION-3.0. STAR files of both Type2-1 and Type2-2 with the metadata of the final refined segments are included.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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