CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to latent TGF-β on a holey carbon grid (strongly preferred orientations) [multiple data sets in TIFF, MRC and MRCS formats]
Publication:
Cryo-EM Reveals Integrin-Mediated TGF-β Activation without Release from Latent TGF-β
αVβ8 integrin bound to latent TGF-β on holey carbon grids (particles have strongly preferred orientations): 1684 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 16 second movies contain 80 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~70 e/Å^2. The gain reference, a file containing coordinates for a single dead column, and a list of the 1364 micrographs that were used for downstream processing are included.
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αVβ8 integrin bound to latent TGF-β on holey carbon grids (particles have strongly preferred orientations): 1684 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~70 e/Å^2. A list of the 1364 micrographs that were used for downstream processing is included.
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αVβ8 integrin bound to latent TGF-β on holey carbon grids (particles have strongly preferred orientations): Stacks of 68,356 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after extensive 2D- and 3D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. This particle stack was combined with a stack of particles collected on graphene oxide to complement preferred orientations. The graphene oxide particle stack is deposited with the accession number EMPIAR-10344.
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Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
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