EMPIAR-10338 - Rabbit muscle aldolase movies obtained using a Talos Arctica (200 kV) equipped with a K2 [3316 multi-frame micrographs composed of 44 frames each in TIFF format]

EMPIAR-10338

Rabbit muscle aldolase movies obtained using a Talos Arctica (200 kV) equipped with a K2 [3316 multi-frame micrographs composed of 44 frames each in TIFF format]

Publication:

Sub-2 Angstrom resolution structure determination using single-particle cryo-EM at 200 keV

Wu M, Lander GC, Herzik MA

Journal of Structural Biology: X 4 (2020)

DOI: 10.1016/j.yjsbx.2020.100020

Related EMDB entry:

EMD-21023

Deposited:

2019-08-30

Released:

2020-04-24

Last modified:

2020-04-24

Imageset size:

583.40 GB

Imageset DOI:

10.6019/EMPIAR-10338

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Rabbit muscle aldolase movies obtained using a Talos Arctica (200 kV) equipped with a K2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3316

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.91, 0.91)

Details:

Unaligned counting mode frame stacks (44 frames/movie) in compressed TIFF file format of rabbit muscle aldolase in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Two separate data collections (17jul27d and 17aug25c) that were combined are provided. Images used for corrections for each data set are also included (require flip along Y axis).

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

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Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

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