EMPIAR-10063 - Cryo-EM Structure of the Activated NAIP2/NLRC4 Inflammasome Reveals Nucleated Polymerization [multiple data sets in SPIDER and MRC formats]

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EMPIAR-10063

Cryo-EM Structure of the Activated NAIP2/NLRC4 Inflammasome Reveals Nucleated Polymerization [multiple data sets in SPIDER and MRC formats]

Publication:

Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization

Zhang L, Chen S, Ruan J, Wu J, Tong AB, Yin Q, Li Y, David L, Lu A, Wang WL, Marks C, Ouyang Q, Zhang X, Mao Y, Wu H

Science (New York, N.Y.) 350 (2015) 404-409

PMID: 26449474

DOI: 10.1126/science.aac5789

Related EMDB entry:

EMD-6458 EMD-6460 EMD-6459

Deposited:

2016-06-13

Released:

2016-06-17

Last modified:

2016-06-17

Imageset size:

1.69 TB

Imageset DOI:

10.6019/EMPIAR-10063

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Drift-corrected micrographs of NLRC4/NAIP2 inflammasome

Category:

micrographs - single frame

Image format:

SPIDER

No. of images or tilt series:

7861

Image size:

(7420, 7676)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.86, 0.86)

Details:

The micrographs are the average after sub-frame based drift correction of 36 raw movie frames (47.6 electrons per squared Angstrom in 9 second exposure).

Files:

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2. Single-particle stacks of NLRC4/NAIP2 inflammasome

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

110033

Image size:

(448, 448)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.86, 0.86)

Details:

ring10.mrcs contains 4,489 particles, with 250*250 image size, 1.72*1.72 pixel size. ring11.mrcs contains 75,114 particles, with 448* 448 image size, 0.86*0.86 pixel size. ring12.mrcs contains 30,430 particles, with 250*250 image size, 1.72*1.72 pixel size.

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