EMPIAR-12793
CryoEM dataset of Retron-Eco1 WT filaments in the presence of NAD+ [multiple data sets in MRC format]
Publication:

Retron-Eco1 assembles NAD+ hydrolyzing filaments that provide immunity against bacteriophages

Carabias A, Camara-Wilpert S, Mestre MR, Lopéz-Méndez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G

Molecular cell 84 (2024) 2185-2202000000000000.0

PMID: 38788717

Related EMDB entry:
Deposited:
2025-05-21
Released:
2025-07-01
Last modified:
2025-07-01
Imageset size:
9.87 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multiframe micrographs of Retron-Eco1 WT in presence of NAD+ (Dataset1-acdr0162)
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2669
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.832, 0.832)
Details:
Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 96000 (0.832 A˚ /px) using a total dose of 39 e/A˚ 2. Spherical Aberration: 2.7 mm. Micrographs are gain-corrected, non-motion-corrected.

Microscope Settings:
-Acceleration Voltage: 300 kV
-Pixel Size: 0.832 Å
-Spherical Aberration: 2.7 mm
-Total Exposure Dose: 39 e/Ų
-Number of Fractions: 40
Files:
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2. Unaligned multiframe micrographs of Retron-Eco1 WT in presence of NAD+ (Dataset2-acdr0168)
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
5197
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.832, 0.832)
Details:
Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 96000 (0.832 A˚ /px) using a total dose of 39 e/A˚ 2. Spherical Aberration: 2.7 mm. Micrographs are gain-corrected, non-motion-corrected.

Microscope Settings:
-Acceleration Voltage: 300 kV
-Pixel Size: 0.832 Å
-Spherical Aberration: 2.7 mm
-Total Exposure Dose: 39 e/Ų
-Number of Fractions: 40
Files:
Loading...
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