EMPIAR-12791
CryoEM dataset of Cas12j3 (Casphi3) in complex with crRNA and target DNA after cleavage (postcatalytic state) [4445 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

Carabias A, Fuglsang A, Temperini P, Pape T, Sofos N, Stella S, Erlendsson S, Montoya G

Nature communications 12 (2021) 4476-na

PMID: 34294706

Related EMDB entry:
Deposited:
2025-05-08
Released:
2025-07-01
Last modified:
2025-07-01
Imageset size:
5.58 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame movies of Cas12j3/crRNA/DNA complex in postcleavage state.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
4445
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.832, 0.832)
Details:
Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 96000 (0.832 A˚ /px) using a total dose of 42 e/A˚ 2. Spherical Aberration: 2.7 mm. Out of the 4445 original micrographs deposited (gain-corrected, non-motion-corrected, 40 fractions), 4393 were used for picking.

Microscope Settings:
-Acceleration Voltage: 300 kV
-Pixel Size: 0.832 Å
-Spherical Aberration: 2.7 mm
-Total Exposure Dose: 42 e/Ų
-Number of Fractions: 40
Files:
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