Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×150,000 nominal magnification with a pixel size of 0.952 Å. A total of 3490 movies were collected in 30 frames with a total dose of 30.97 e−/Å2 (1.01 e−/pixel/s) over 27.79 s with a set defocus between−0.6 and−1.4 µm. The movies are gain corrected. All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.
Collected on a Titan Krios G2 operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at ×105,000 nominal magnification with a calibrated pixel size of 0.8240 Å. A total of 8533 movies were collected in 30 frames with a total dose of 28.52 e−/Å2 (16.1 e−/pixel/s) over 1 s with a set defocus between−0.8 and−1.4 µm. The movies are gain corrected. All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.
Collected on a Titan Krios G3i operated at 300 kV and equipped with a K3 Bio-Quantum direct electron detector and energy lter using 20 eV slit. The data were acquired at ×130,000 nominal magnification with a calibrated pixel size of 0.648 Å. A total of 24,479 movies were collected in 40 frames with a total dose of 40 e−/Å2 (16.8 e−/pixel/s) over 1 s with a set defocus between−0.7 and−1.0 µm. The movies are gain corrected. All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.
Collected on a Titan Krios operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at ×130,000 nominal magnification with a calibrated pixel size of 0.6478 Å. A total of 32,937 were collected in 45 frames with a total dose of 45.47 e−/Å2 (15.9 e−/pixel/s) over 1.2 s with a set defocus between−0.7 and−1.0 µm. The movies are not gain corrected. The gain file is provided. All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.
Collected on a Titan Krios G2 operated at 300 kV and equipped with a Falcon-4i direct electron detector and Selectris energy filter using 10 eV slit. The data were acquired at ×165,000 nominal magnification with a calibrated pixel size of 0.728 Å. A total of 10,065 EER formatted exposures were collected in 657 raw frames with a total dose of 28.14 e−/Å2 (14.06 e−/pixel/s) over 2.14 s with a set defocus between −0.7 and−1.3 µm. The movies are not gain corrected. The gain file is provided. All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.
Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×190,000 nominal magnification with a pixel size of 0.7463 Å. A total of 1318 movies were collected in 45 frames with a total dose of 42.91 e−/Å2 (0.86 e−/pixel/s) over 27.79 s with a set defocus between−0.5 and−1.5 µm. The movies are gain corrected.
Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×190,000 nominal magnification with a pixel size of 0.7463 Å. A total of 5489 movies were collected in 46 frames with a total dose of 48.40 e−/Å2 (1.00 e−/pixel/s) over 28.41 s with a set defocus between−0.5 and−1.5 µm. The movies are gain corrected.
Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×150,000 nominal magnification with a pixel size of 0.952 Å. A total of 4393 movies were collected in 31 frames with a total dose of 29.8 e−/Å2 (0.94 e−/pixel/s) over 28.74 s with a set defocus between−0.5 and−1.5 µm. The movies are gain corrected.