EMPIAR-12695
CryoEM of purified immature tick borne encephalitis virus particles, strain Kuutsalo-14 [multiple data sets in TIFF format]
Publication:

The structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation

Anastasina M, Füzik T, Domanska A, Pulkkinen LIA, Šmerdová L, Formanová PP, Straková P, Nováček J, Růžek D, Plevka P, Butcher SJ

Science advances 10 (2024) eadl1888-eadl1888

PMID: 38959313

Related EMDB entry:
Deposited:
2024-08-02
Released:
2025-05-27
Last modified:
2025-05-27
Imageset size:
26.66 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of immature TBEV (Kuutsalo-14) stored in *.tar.gz archives, data set 1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
26990
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.1041, 1.1041)
Details:
Microscope Details:
Accelerating voltage: 300 kV
Spherical aberration: 2.7 mm

Data acquisition details:
Nominal Magnification: 81,000X
Pixel Size: 1.1041Å
Dose Rate: 16 e/px/s
Exposure: 3.3s (247/75)
Total Dose: ~43e/Ų
Number of Frames: 50
Target Defocuses: -0.7um to -2.7um at 0.2um step
File format: Gain Normalized LZW compressed TIFF
Files:
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2. Unaligned multi-frame micrographs of immature TBEV (Kuutsalo-14) stored in *.tar.gz archives, data set 2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
24271
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.0859, 1.0859)
Details:
Microscope Details:
Accelerating voltage: 300 kV
Spherical aberration: 2.7 mm

Data acquisition details:
Nominal Magnification: 81000 X
Defocus Range: -0.6 um to -2.0 um
Defocus Step: 0.2 pm
Dose Rate: 19.514 e/px/s
Dose Rate: 16.549 e/square angstrom⁄s
Exposure Time: 2.6 s
Total Dose: 43.027 e/A2
Dose Fractions (type): 50 (Physical Pixel TIFF LZW Gain normalized)
Dose per Fraction: 0.861 e/square angstrom

Software: EPU v2.12.1
Selection Type: Automated
Acquisition Mode: Faster Acquisition
Files:
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