EMPIAR-12607
Cryo-EM of SARS-CoV-2 pre-incorporation replication-transcription complex with ATP [11657 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:

Structural basis for substrate selection by the SARS-CoV-2 replicase

Malone BF, Perry JK, Olinares PDB, Lee HW, Chen J, Appleby TC, Feng JY, Bilello JP, Ng H, Sotiris J, Ebrahim M, Chua EYD, Mendez JH, Eng ET, Landick R, Götte M, Chait BT, Campbell EA, Darst SA

Nature 614 (2023) 781-787

PMID: 36725929

Related EMDB entry:
Deposited:
2025-03-06
Released:
2025-04-07
Last modified:
2025-04-07
Imageset size:
2.81 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. unaligned raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
11657
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.08, 1.08)
Details:
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 21.4 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 52 e-/A^2; pixel size 1.08 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
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