EMPIAR-12600
Ensemble cryo-EM reveals conformational states of the nsp13 helicase replication-transcription complex [multiple data sets in TIFF format]
Publication:

Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex

Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA

Nature structural & molecular biology 29 (2022) 250-260

PMID: 35260847

Deposited:
2025-02-28
Released:
2025-04-24
Last modified:
2025-04-24
Imageset size:
5.27 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. unaligned raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4358
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.07, 1.07)
Details:
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 24.7 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 64 e-/A^2; pixel size 1.1 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
Files:
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2. unaligned raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
13448
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.07, 1.07)
Details:
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 24.7 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 64 e-/A^2; pixel size 1.1 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
Files:
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