EMPIAR-12598
Cryo electron microscopy of backtracked SARS-CoV-2 nsp13-replication-transcription complex [10685 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:

Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex

Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 33883267

Related EMDB entry:
Deposited:
2025-02-27
Released:
2025-04-24
Last modified:
2025-04-24
Imageset size:
3.96 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. unaligned raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10685
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.065, 1.065)
Details:
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 30 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 26.4 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 66 e-/A^2; pixel size 1.065 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.
Files:
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