EMPIAR-12444
Single particle reconstruction of E. coli TnsB in complex with right end fragment of Tn7 transposon [multiple data sets in TIFF and MRCS formats]
Publication:

Structural basis of transposon end recognition explains central features of Tn7 transposition systems

Kaczmarska Z, Czarnocki-Cieciura M, Górecka-Minakowska KM, Wingo RJ, Jackiewicz J, Zajko W, Poznański JT, Rawski M, Grant T, Peters JE, Nowotny M

Molecular cell 82 (2022) 2618-2632.e7

PMID: 35654042

Related EMDB entry:
Deposited:
2024-11-02
Released:
2025-02-03
Last modified:
2025-02-03
Imageset size:
4.12 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame movies of E. coli TnsB in complex with right end fragment of Tn7 transposon
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6692
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.86, 0.86)
Details:
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 40.16 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.0, -1.7, -1.4, -1.1, -0.8, -0.5 µm. Objective aperture retracted, C2 aperture: 50 µm.
Files:
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2. Aligned particles of E. coli TnsB in complex with right end fragment of Tn7 transposon (helical reconstruction)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
450748
Image size:
(512, 512)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.86, 0.86)
Details:
Aligned particles with corresponding Relion-3 STAR file for helical reconstruction. Coordinates correspond to micrographs binned 2x during Motion correction.
Files:
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3. Aligned particles of E. coli TnsB in complex with right end fragment of Tn7 transposon (single particle reconstruction)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
901496
Image size:
(512, 512)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.86, 0.86)
Details:
Aligned particles with corresponding Relion-3 STAR file for single particle reconstruction. Particles were signal-subtracted; coordinates correspond to micrographs binned 2x during Motion correction.
Files:
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