micrographs - multiframe

TIFF

15690

(5760, 4092)

UNSIGNED BYTE

(0.934, 0.934)

Data for grid 1, collected at the MRC LMB Krios1

Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 81000
Pixel size 0.934 A/pix
Dose 40 e/A2
Exposure rate 25 e/pix/s
Exposure time 1.44s
Fractions 40 by mistake, some of the movies have 41 frames instead. Depending on your processing strategy, these might have to be removed or processed seperately to avoid bugs.

The gain reference "gainref.tiff" is provided. It can be used without rotation or flipping for RELION motion correction.

Metadata are also included for all maps deposited to the EMDB from this dataset. These are direct copies of the "run_data.star" from the Refine3D directory of the RELION project.

Download #files

micrographs - multiframe

TIFF

35423

(5760, 4092)

UNSIGNED BYTE

(0.664, 0.664)

Data for grid 2, collected at the MRC LMB Krios3

Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 130000
Pixel size 0.664 A/pix
Dose 40 e/A2
Exposure rate 25 e/pix/s
Exposure time 0.72s
Fractions 40

The gain reference "gainref.tiff" is provided. It can be used without rotation or flipping for RELION motion correction.

Metadata are also included for all maps deposited to the EMDB from this dataset. These are direct copies of the "run_data.star" from the Refine3D directory of the RELION project.

Download #files