EMPIAR-12161
SBF SEM images of a notoroid in transverse orientation (Fig S24) [multiple data sets in DM4 and TIFF formats]
Publication:

Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation

Tiago Rito TR, Ashley R.G. Libby ARGL, Madeleine Demuth MD, Marie-Charlotte Domart MCD, Jake Cornwall-Scoones JCS, James Briscoe JB

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Related EMDB entry:
Deposited:
2024-02-14
Released:
2024-09-26
Last modified:
2024-09-26
Imageset size:
11.54 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Low magnification raw SBF SEM overview images (ROI00) of notoroid in transverse orientation
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
50
Image size:
(7000, 7000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(610.0, 610.0)
Details:
Low magnification overview SBF SEM images (ROI00), linked to higher magnification images in ROI01 and ROI02 below. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.
Files:
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2. High magnification raw SBF SEM images (ROI01) of notochord in notoroid in transverse orientation
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
50
Image size:
(7000, 7000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(88.0, 88.0)
Details:
High magnification images (ROI01) linked to lower magnification images in ROI00. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.
Files:
Loading...
3. High magnification raw SBF SEM images (ROI02) of notochord in notoroid in transverse orientation
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
50
Image size:
(7000, 7000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(83.0, 83.0)
Details:
High magnification images (ROI02) linked to lower magnification images in ROI00. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.
Files:
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4. Survey image showing the relative position of each of the ROIs above
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Survey image used to setup the multi ROI run in Digital Micrograph. Shows the position of each ROI.
Files:
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