EMPIAR-11946
Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells [multiple data sets in MRC format]
Publication:

Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Khusainov I, Romanov N, Goemans C, Turoňová B, Zimmerli CE, Welsch S, Langer JD, Typas A, Beck M

Nature communications 15 (2024)

PMID: 38839776

Related EMDB entry:
Deposited:
2024-03-07
Released:
2024-06-19
Last modified:
2024-06-19
Imageset size:
35.83 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
1. Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells dataset 1
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
6
Image size:
(5760, 4092)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.697, 1.697)
Details:
The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -8 degree pre-tilt and covering a range from -64 to +48 degrees, with a tilt increment of 2 degrees. Each wedge includes 4 tilts.
Files:
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2. Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells dataset 2
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
13
Image size:
(5760, 4092)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.697, 1.697)
Details:
The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -8 degree pre-tilt and covering a range from -63 to +45 degrees, with a tilt increment of 2 degrees. Each wedge includes 4 tilts.
Files:
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Files:
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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
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