EMPIAR-11904
Single particle cryo-EM of ASCT2 in lipid nanodiscs with bound glutamine and Na+ ions [multiple data sets in TIFF format]
Publication:

Structural basis of the obligatory exchange mode of human neutral amino acid transporter ASCT2

Borowska AM, Chiariello MG, Garaeva AA, Rheinberger J, Marrink SJ, Paulino C, Slotboom DJ

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Deposited:
2024-02-01
Released:
2025-04-24
Last modified:
2025-04-24
Imageset size:
3.36 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned movie frames of ASCT2 in lipid nanodiscs in the presence of glutamine and Na+ ions (dataset 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1698
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Data represents unaligned multi-frame movies. Each movie contains 76 frames collected from the Quantifoil grid at 300 kV in counting mode on a K3 camera. Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.
Files:
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2. Unaligned movie frames of ASCT2 in lipid nanodiscs in the presence of glutamine and Na+ ions (dataset 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
752
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Data represents unaligned multi-frame movies. Each movie contains 76 frames collected from the Quantifoil grid at 300 kV in counting mode on a K3 camera. Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.
Files:
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3. Unaligned movie frames of ASCT2 in lipid nanodiscs in the presence of glutamine and Na+ ions (dataset 3)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2447
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Data represents unaligned multi-frame movies. Each movie contains 76 frames collected from the Quantifoil grid at 300 kV in counting mode on a K3 camera. Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.
Files:
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4. Unaligned movie frames of ASCT2 in lipid nanodiscs in the presence of glutamine and Na+ ions (dataset 4)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
128
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Data represents unaligned multi-frame movies. Each movie contains 76 frames collected from the Quantifoil grid at 300 kV in counting mode on a K3 camera. Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.
Files:
Loading...
5. Unaligned movie frames of ASCT2 in lipid nanodiscs in the presence of glutamine and Na+ ions (dataset 5)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1645
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Data represents unaligned multi-frame movies. Each movie contains 76 frames collected from the Quantifoil grid at 300 kV in counting mode on a K3 camera. Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.
Files:
Loading...
6. Unaligned movie frames of ASCT2 in lipid nanodiscs in the presence of glutamine and Na+ ions (dataset 6)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
148
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Data represents unaligned multi-frame movies. Each movie contains 76 frames collected from the Quantifoil grid at 300 kV in counting mode on a K3 camera. Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.
Files:
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7. Unaligned movie frames of ASCT2 in lipid nanodiscs in the presence of glutamine and Na+ ions (dataset 7)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1912
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Data represents unaligned multi-frame movies. Each movie contains 75 frames collected from the Quantifoil grid at 300 kV in counting mode on a K3 camera. Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.
Files:
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