reconstructed volumes

PNG

1376

(5092, 5526)

UNSIGNED BYTE

(10.0, 10.0)

Voxel size: 10x10x40 nm

SBF-SEM imaging of a larval cheatal sac at 48 hours post fertilisation.
These files show one of the cheatoblasts constructing the blade region
of a chaeta
Files:
Dataset1_200827_Blade_48hpf.am -> full imaged stack
Dataset1_200827_Blade_48hpf_crop.am -> crop from the above full stack with the model:
Labels_Dataset1_200827_Blade_48hpf_crop.model -> Model in MIB format (https://mib.helsinki.fi/)
Labels_Dataset1_200827_Blade_48hpf_crop.tif -> Model in TIF format
Labels Ids:
0 - background
1 - channels (green in the snapshot)
2 - shell (brown in the snapshot)

Additional information:
Source for movie "S3_SBF-SEM_Reconstruction_Blade" from the manuscript
Dynamic microvilli sculpt bristles at nanometric scale by
Kyojiro N Ikeda, Ilya Belevich, Luis Zelaya-Lainez, Lukas Orel, Josef Füssl,
Jaromír Gumulec, Christian Hellmich, Eija Jokitalo, Florian Raible

Download #files

reconstructed volumes

PNG

936

(4355, 6026)

UNSIGNED BYTE

(10.0, 10.0)

Voxel size: 10x10x40 nm

SBF-SEM imaging of a larval cheatal sac at 48 hours post fertilisation.
These files show one of the cheatoblasts constructing the joint region
of a chaeta

Files:
Dataset2_200916_Joint_48hpf.am -> full imaged stack
Dataset2_200916_Joint_48hpf_crop.am -> crop from the above full stack with the model:
Labels_Dataset2_200916_Joint_48hpf_crop.model -> Model in MIB format (https://mib.helsinki.fi/)
Labels_Dataset2_200916_Joint_48hpf_crop.tif -> Model in TIF format
Labels Ids:
0 - background
1 - cavity in the shaft (blue in the snapshot)
2 - channels (green in the snapshot)

Labels_Dataset2_200916_Joint_48hpf_crop_shell.model
Labels_Dataset2_200916_Joint_48hpf_crop_shell.tif
Labels Ids:
0 - background
1 - shell (brown in the snapshot)

Additional information:
Source for movie "S5_SBF-SEM_Reconstruction_Joint" from the manuscript
Dynamic microvilli sculpt bristles at nanometric scale by
Kyojiro N Ikeda, Ilya Belevich, Luis Zelaya-Lainez, Lukas Orel, Josef Füssl,
Jaromír Gumulec, Christian Hellmich, Eija Jokitalo, Florian Raible

Download #files

reconstructed volumes

PNG

1071

(3388, 3954)

UNSIGNED BYTE

(10.0, 10.0)

Voxel size: 10x10x40 nm

SBF-SEM imaging of a larval cheatal sac at 64 hours post fertilisation.
These files show one of the cheatoblasts constructing the shaft region of a chaeta

Files:
Dataset3_211020_Shaft_64hpf.am -> full imaged stack
Dataset3_211020_Shaft_64hpf_crop.am -> crop from the above full stack with the model:
Labels_Dataset3_211020_Shaft_64hpf_crop.model -> Model in MIB format (https://mib.helsinki.fi/)
Labels_Dataset3_211020_Shaft_64hpf_crop.tif -> Model in TIF format
Labels Ids:
0 - background
1 - channels (green in the snapshot)
2 - amorphous material inside the cavity of the shaft (yellow in the snapshot)
3 - cavity in the shaft (blue in the snapshot)

Labels_Dataset3_211020_Shaft_64hpf_shell.model
Labels_Dataset3_211020_Shaft_64hpf_shell.tif
Labels Ids:
0 - background
1 - shell (brown in the snapshot)

Additional information:
Source for movie S7_SBF-SEM_Reconstruction_Shaft" from the manuscript
Dynamic microvilli sculpt bristles at nanometric scale by
Kyojiro N Ikeda, Ilya Belevich, Luis Zelaya-Lainez, Lukas Orel, Josef Füssl,
Jaromír Gumulec, Christian Hellmich, Eija Jokitalo, Florian Raible

Download #files