EMPIAR-11762
Cryo-EM structure of human TFIIIC bound and unbound to tRNA gene [multiple data sets in TIFF and MRCS formats]
Publication:

Structural insights into human TFIIIC promoter recognition

Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Müller CW

Science advances 9 (2023)

PMID: 37418517

Deposited:
2023-11-02
Released:
2024-02-28
Last modified:
2024-02-28
Imageset size:
4.14 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame micrographs of human TFIIIC bound to tRNA gene
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
11025
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.822, 0.822)
Details:
Unaligned multi-frame (40 frames) micrographs were collected in counting mode with a total electron dose of 42.8 electrons/Å2 at defocus range from 0.7 to 1.7 . Gain reference is in CountRef_211108_hTFIIIC_DNA_00001.mrc file.
Files:
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2. Final particles of TauA subcomplex
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
55079
Image size:
(352, 352)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
The directory "data/Dataset_1/TauA_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauA. These particles were also used for the "Cryo-EM mapping of particle pair distances at a single-molecule level" with TauB-dimer particles. The file "particles.star" contains the coordinates.
Files:
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3. Final particles of TauB-dimer subcomplex
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
35379
Image size:
(480, 480)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
The directory "data/Dataset_1/TauB_dimer_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauB-dimer. The file "particles.star" contains the coordinates.
Files:
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4. Intermediate particles of TauB-dimer subcomplex for mapping pair distance
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
99217
Image size:
(480, 480)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
The directory "data/Dataset_1/TauB_dimer_pair_distances" contains the ".mrcs" files of Non-Uniform refinement reconstruction of TauB-dimer. These particles were used for the "Cryo-EM mapping of particle pair distances at a single-molecule level" with TauA particles. The file "particles.star" contains the coordinates.
Files:
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5. Unaligned multi-frame micrographs of human TFIIIC (No DNA sample)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
11088
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.822, 0.822)
Details:
Unaligned multi-frame (40 frames) micrographs were collected in counting mode with a total electron dose of 39.6 electrons/Å2 at defocus range from 0.7 to 1.7 . Gain reference is in CountRef_hTFIIIC_OG_113-1_00001.mrc file.
Files:
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6. Final particles of TauA subcomplex (No DNA sample)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
94103
Image size:
(300, 300)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
The directory "data/Dataset_2/TauA_noDNA_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauA. The file "particles.star" contains the coordinates.
Files:
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7. Final particles of TauB-dimer subcomplex (No DNA sample)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
125600
Image size:
(480, 480)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
The directory "data/Dataset_2/TauB_dimer_noDNA_final_particles" contains the ".mrcs" files of the final 3D reconstruction of TauA. The file "particles.star" contains the coordinates.
Files:
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