EMPIAR-11747
In situ cryo-electron tomography of the T. pseudonana diatom PyShell [multiple data sets in MRC and TIFF formats]
Publication:

Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation

Shimakawa G, Demulder M, Flori S, Kawamoto A, Tsuji Y, Nawaly H, Tanaka A, Tohda R, Ota T, Matsui H, Morishima N, Okubo R, Wietrzynski W, Lamm L, Righetto RD, Uwizeye C, Gallet B, Jouneau PH, Gerle C, Kurisu G, Finazzi G, Engel BD, Matsuda Y

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Related EMDB entry:
Deposited:
2023-10-20
Released:
2024-10-08
Last modified:
2024-10-08
Imageset size:
81.81 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • reconstructed volumes
  • tilt series
  • micrographs - multiframe
1. Denoised bin4 tomograms
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
7
Image size:
(1024, 1440)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(8.572, 8.572)
Details:
Tomograms denoised using cryo-CARE trained on each individual tomogram reconstructed from odd/even frames.
Files:
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2. IMOD bin4 tomograms
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
7
Image size:
(1024, 1440)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(8.572, 8.572)
Details:
IMOD reconstruction at bin4 using weighted backprojection without CTF correction. IMOD reconstruction files (.xf and .tlt) are provided in the imod_files/ directory.
Files:
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3. Tilt series assembled from motion-corrected and averaged frames
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
7
Image size:
(5760, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.143, 2.143)
Details:
Frames aligned with MotionCor2
Note: Frames per image varies
Files:
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4. Raw frames
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
327
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.071, 1.071)
Details:
Movies acquired with a K3 detector in super-resolution mode using SerialEM. Gain reference and defects file are provided in the gainref/ directory. Metadata from the acquisition can be found in the mdocs/ directory.
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)