EMPIAR-11743
Cryo-EM SPA dataset of stably reduced Streptococcus pneumoniae NADPH oxidase with NADH [28120 multi-frame micrographs composed of 938 frames each in EER format]
Publication:

Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase

Dubach VRA, San Segundo-Acosta P, Murphy BJ

Nature structural & molecular biology (2024)

PMID: 39039317

Related EMDB entry:
Deposited:
2023-10-16
Released:
2025-06-26
Last modified:
2025-06-26
Imageset size:
12.18 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned mutliframe non-gain-normalized EER movies of SpNOX collected on a Krios G4
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
28120
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.573, 0.573)
Details:
Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.

The EER movies contain 938 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.

XML files to generate optics groups are provided as well as the gain reference.
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