EMPIAR-11656
Single particle cryo-EM dataset of mitochondrial complex I from Mus musculus inhibited by IACS-2858 - 2 [571 multi-frame micrographs composed of 25 frames each in MRC format]
Publication:

Cork-in-bottle mechanism of inhibitor binding to mammalian complex I.

Chung I, Serreli R, Cross JB, Di Francesco ME, Marszalek JR, Hirst J

Science advances 7 (2021)

PMID: 33990335

Related EMDB entry:
Deposited:
2023-08-04
Released:
2023-10-03
Last modified:
2023-10-03
Imageset size:
95.94 GB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   793,990,636 bytes
Compressed size: 100,599,527 bytes
Contains:
  • micrographs - multiframe
1. Unaligned, gain-corrected micrographs of mitochondrial complex I from mouse heart inhibited with IACS-2858
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
571
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.055, 1.055)
Details:
Micrographs are the average of 25 unaligned, gain-corrected frames. Images were collected on a Krios with a K2 detector and energy filter with a slit width of 20eV. Data were collected over 10 seconds with a dose rate of 5.84 e / A-2 / s with a total dose of 58.4 e/A-2.
Files:
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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)