EMPIAR-11309
Cryo-EM structure of the wild-type solitary ECF module in MSP2N2 lipid nanodiscs in the ATPase open and nucleotide-free conformation (200 kV) [multiple data sets in TIFF format]
Publication:

Expulsion mechanism of the substrate-translocating subunit in ECF transporters

Thangaratnarajah C, Nijland M, Borges-Araújo L, Jeucken A, Rheinberger J, Marrink SJ, Souza PCT, Paulino C, Slotboom DJ

Nature communications 14 (2023)

PMID: 37491368

Related EMDB entry:
Deposited:
2022-11-15
Released:
2023-08-18
Last modified:
2023-08-18
Imageset size:
1.89 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned movie frames (Dataset 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1400
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.022, 1.022)
Details:
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.
Files:
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2. Unaligned movie frames (Dataset 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4205
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.022, 1.022)
Details:
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.
Files:
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3. Unaligned movie frames (Dataset 3)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
60
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.022, 1.022)
Details:
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.
Files:
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4. Unaligned movie frames (Dataset 4)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2981
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.022, 1.022)
Details:
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.
Files:
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