EMPIAR-10922
Cryo-electron tomographs of mouse thalamus neurons [60 reconstructed volumes in MRC format]
Publication:

In situ cryo-electron tomography reveals local cellular machineries for axon branch development

Nedozralova H, Basnet N, Ibiricu I, Bodakuntla S, Biertümpfel C, Mizuno N

The Journal of cell biology 221 (2022)

PMID: 35262630

Related EMDB entry:
Deposited:
2022-01-10
Released:
2022-03-04
Last modified:
2022-10-10
Imageset size:
71.14 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • reconstructed volumes
1. Reconstructed 4x binned tomograms of thalamus neurons.
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
60
Image size:
(928, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(21.8, 21.8)
Details:
Tilt-series were filtered according to the cumulative radiation dose (Grant and Grigorieff, 2015) and aligned on the basis of the patch tracking algorithm using the IMOD ETOMO package (Kremer et al., 1996). Tomograms were reconstructed from aligned stacks using weighted back-projection in IMOD. Tomograms were further 4x binned, resulting in pixel sizes of 21.8 Å. Tomograms were denoised by edge enhancing diffusion (bnad command in Bsoft (Heymann et al., 2008)).
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