EMPIAR-10915
CryoEM data of PLA2R at pH 6.2 with both 0 and 30 degree tilts. [multiple data sets in TIFF format]
Publication:

Structure of PLA2R reveals presentation of the dominant membranous nephropathy epitope and an immunogenic patch

Fresquet M, Lockhart-Cairns MP, Rhoden SJ, Jowitt TA, Briggs DC, Baldock C, Brenchley PE, Lennon R

Proceedings of the National Academy of Sciences of the United States of America 119 (2022)

PMID: 35858348

Related EMDB entry:
Deposited:
2020-06-17
Released:
2022-07-12
Last modified:
2022-10-10
Imageset size:
2.13 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame images of PLA2R at pH 6.2 with a 30 degree tilt.
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2523
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.415, 0.415)
Details:
Images were collected at 30 degree tilt on a K3 in super res mode with sampling of 0.415 Å/pix. The gain image is in its own directory.
Titan Krios: 300 kV
Cs: 2.7
Frames: 40
Dose: 42.9 e/Å^2
Dose rate: 14.8 e.pix/sec
Files:
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2. Unaligned multi-frame images of PLA2R at pH 6.2 with 0 degree tilt (not used in publication)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3703
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.415, 0.415)
Details:
Images were collected at 0 degree tilt on a K3 in super res mode with sampling of 0.415 Å/pix. The gain image is in the main directory. This data was not used in the final publication.
Titan Krios: 300 kV
Cs: 2.7
Frames: 40
Dose: 42.9 e/Å^2
Dose rate: 14.8 e.pix/sec
Files:
Loading...
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