EMPIAR-10907
FIB-SEM of an Mbp-deficient shiverer mouse optic nerve [857 micrographs in TIFF format]
Publication:

White matter integrity in mice requires continuous myelin synthesis at the inner tongue

Meschkat M, Steyer AM, Weil MT, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Phan NTN, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave KA, Möbius W

Nature communications 13 (2022)

PMID: 35246535

Related EMDB entry:
Deposited:
2021-12-17
Released:
2022-01-12
Last modified:
2022-10-17
Imageset size:
18.84 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. FIB-SEM data stack of an Mbp-deficient shiverer mouse optic nerve
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
857
Image size:
(4650, 5076)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(50.0, 50.0)
Details:
Optic nerve of a male Mbp-deficient mouse (shiverer), perfusion fixation: 2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl, rOTO embedding, Durcupan.
FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 700 pA, z-step 50 nm
Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ
Files:
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