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Electron Microscopy Public Image Archive

EMPIAR-10823
Cryo electron micrographs of Pol delta-PCNA-DNA-FEN1 sample [5073 multi-frame micrographs composed of 45 frames each in TIFF format]
Publication:

Structure of the processive human Pol δ holoenzyme

Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A

Nature communications 11 (2020)

PMID: 32111820

Related EMDB entry:
EMD-10539 EMD-10540
Deposited:
2021-08-19
Released:
2021-10-22
Last modified:
2021-10-22
Imageset size:
1.73 TB
Imageset DOI:
10.6019/EMPIAR-10823
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. Cryo-electron micrographs of Pol delta-FEN1 toolbelt
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5073
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.435, 0.435)
Details:
Images taken using a FEI Titan Krios G3 with Gatan K3 camera in super resolution mode (binning 1). Defocus range -2.3 to -1.1. Total dose = 43.6 e-/Å^2. Dataset contains 743 corrupted or partially corrupted movies, as file names suggest - these became corrupted after the deposited EMDB maps were generated. The given pixel size accounts for super resolution binning 1 - the physical pixel size was 0.87 Å/pix.
Files:
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