EMPIAR-10593
Nanoscale view of the Clostridium thermocellum cellulosome during cellulose degradation reveals an ecological strategy leading to phenotypic heterogeneity [37 tilt series in MRC format]
Publication:

Nanoscale view of the Clostridium thermocellum cellulosome during cellulose degradation reveals an ecological strategy leading to phenotypic heterogeneity

Tatli M, Moraïs S, Tovar-Herrera OE, Bomble Y, Bayer EA, Medalia O, Mizrahi I

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Related EMDB entry:
Deposited:
2020-12-14
Released:
2021-01-08
Last modified:
2022-02-08
Imageset size:
1004.34 MB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
1. Aligned tilt series of C.thermocellum grown in MCC for 15 hr
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
37
Image size:
(3708, 3838)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.35, 1.35)
Details:
Includes
a tilt-series that was acquired at a dose-fractionated frame rate of 1
frame per 0.2 seconds, at a magnification of 105,000 x with 0.135 nm pixel
size, and recorded with a 3° increment over a -48° to 60° angular range
(accumulating ~140 e-/Å2) at a -4 defocus.
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