EMPIAR-10588
Cryo-EM reconstruction of heavy chain mouse apoferritin, 8 datasets testing dose rate and correlative double sampling mode for the Gatan K3 direct electron detector. [1143 multi-frame micrographs composed of 120 frames in LZW compressed TIFF format [multiple data sets in TIFF format]
Publication:

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

Sun M, Azumaya CM, Tse E, Bulkley DP, Harrington MB, Gilbert G, Frost A, Southworth D, Verba KA, Cheng Y, Agard DA

Journal of structural biology 213 (2021)

PMID: 33984504

Related EMDB entry:
Deposited:
2020-11-10
Released:
2021-11-26
Last modified:
2021-11-26
Imageset size:
8.48 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1143
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.417, 0.417)
Details:
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.
Files:
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2. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1143
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.417, 0.417)
Details:
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 105kx nominal magnification collected in standard mode of Gatan K3.
Files:
Loading...
3. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 4 e/p/s in CDS mode from Gatan K3
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1143
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.417, 0.417)
Details:
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 4 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.
Files:
Loading...
4. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 4 e/p/s in CDS mode from Gatan K3
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1152
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.417, 0.417)
Details:
1152 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.
Files:
Loading...
5. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3 at lower magnification
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1080
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.673, 0.673)
Details:
1080 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 64kx nominal magnification collected in CDS mode of Gatan K3.
Files:
Loading...
6. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3 at lower magnification
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1107
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.673, 0.673)
Details:
1107 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 64kx nominal magnification collected in standard mode of Gatan K3.
Files:
Loading...
7. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in CDS mode from Gatan K3 at lower magnification
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1152
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.673, 0.673)
Details:
1152 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 64kx nominal magnification collected in CDS mode of Gatan K3.
Files:
Loading...
8. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in standard mode from Gatan K3 at lower magnification
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1143
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.673, 0.673)
Details:
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 64kx nominal magnification collected in standard mode of Gatan K3.
Files:
Loading...
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