EMPIAR-10548
Cryo-ET of actin bundles induced by full length vinculin [2 tilt series in MRC format]
Publication:

Talin-activated vinculin interacts with branched actin networks to initiate bundles

Rajaa Boujemaa-Paterski RBP, Bruno Martins BM, Matthias Eibauer ME, Charlie Beales CB, Benjamin Geiger BG, Medalia O

eLife 9 (2020)

PMID: 33185186

Related EMDB entry:
Deposited:
2020-11-09
Released:
2020-11-20
Last modified:
2020-11-20
Imageset size:
4.00 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
1. Tomograms of actin bundles induced by full length vinculin
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(1024, 1024)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.7, 1.7)
Details:
The tilt-series were acquired at a dose-fractionated frame rate of 1 frame per 0.2 seconds, at a magnification of 42,000 x with 0.17 nm pixel size, and recorded with a 2° increment over a -60° to 60° angular range (accumulating ~70 electrons per squared Angstrom) at a -4 defocus. The tilt-series were then down-sampled, motion-corrected, contrast transfer function corrected and reconstructed in volumes with a size of 1024x1024x512 voxel (with 8x8 total down-sampling and a voxel size of 13.6 Angstrom).
Files:
Loading...
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)