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EMPIAR-10548
Cryo-ET of actin bundles induced by full length vinculin [2 tilt series in MRC format]
Publication:

Talin-activated vinculin interacts with branched actin networks to initiate bundles

Rajaa Boujemaa-Paterski RBP, Bruno Martins BM, Matthias Eibauer ME, Charlie Beales CB, Benjamin Geiger BG, Medalia O

eLife 9 (2020)

PMID: 33185186

Related EMDB entry:
EMD-10737
Deposited:
2020-11-09
Released:
2020-11-20
Last modified:
2020-11-20
Imageset size:
4.00 GB
Imageset DOI:
10.6019/EMPIAR-10548
Experimental metadata:
Download xml json
Contains:
  • tilt series
Image Sets
1. Tomograms of actin bundles induced by full length vinculin
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(1024, 1024)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.7, 1.7)
Details:
The tilt-series were acquired at a dose-fractionated frame rate of 1 frame per 0.2 seconds, at a magnification of 42,000 x with 0.17 nm pixel size, and recorded with a 2° increment over a -60° to 60° angular range (accumulating ~70 electrons per squared Angstrom) at a -4 defocus. The tilt-series were then down-sampled, motion-corrected, contrast transfer function corrected and reconstructed in volumes with a size of 1024x1024x512 voxel (with 8x8 total down-sampling and a voxel size of 13.6 Angstrom).
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Citations
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Cheng Y, He B, Zeng Q, Zhang F, Han R. (2026)
CryoMV: Structure-Prior-Guided Modeling and Real-Particle Validation of Continuous Conformational Transitions in Cryo-EM
Li F, Dong H, Tang S, Wang X, Zhang C, Ji C, Han R, Zhang F, Wan X. (2026)
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QwenCryoMarker: a universal post-processing framework for contamination-aware particle cleaning.
Sun Y, Zhao J, Xu N, Wang L, Ding W, Li M. (2026)
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