EMPIAR-10490
Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphology [multiple data sets in MRC, BIG DATA VIEWER HDF5 and TIFF formats]
Publication:

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies

Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R

Cell host & microbe (2020)

PMID: 33245857

Related EMDB entry:
Deposited:
2020-06-24
Released:
2020-12-11
Last modified:
2021-10-25
Imageset size:
1.15 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
  • reconstructed volumes
  • micrographs - single frame
1. MOI0.5-24h-Dual axis tilt series
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
202
Image size:
(2024, 2024)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 0.5, 24h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
Files:
Loading...
2. MOI5-12h-Dual axis tilt series
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
178
Image size:
(2024, 2024)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 5, 12h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
Files:
Loading...
3. MOI5-24h-Dual axis tilt series
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
152
Image size:
(2024, 2024)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 5, 24h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
Files:
Loading...
4. MOI5-6h-Dual axis tilt series
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
14
Image size:
(2024, 2024)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 5, 6h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
Files:
Loading...
5. MOI0.5-24h-Reconstructed tomograms
Category:
reconstructed volumes
Image format:
BIG DATA VIEWER HDF5
No. of images or tilt series:
97
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 0.5, 24h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
Files:
Loading...
6. MOI5-12h-Reconstructed tomograms
Category:
reconstructed volumes
Image format:
BIG DATA VIEWER HDF5
No. of images or tilt series:
85
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 5, 12h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
Files:
Loading...
7. MOI5-24h-Reconstructed tomograms
Category:
reconstructed volumes
Image format:
BIG DATA VIEWER HDF5
No. of images or tilt series:
56
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 5, 24h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
Files:
Loading...
8. MOI5-6h-Reconstructed tomograms
Category:
reconstructed volumes
Image format:
BIG DATA VIEWER HDF5
No. of images or tilt series:
7
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(15.578, 15.578)
Details:
Condition: MOI = 5, 6h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
Files:
Loading...
9. S4 Area 2 FIB-SEM Volume 8nm isotropic
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
4824
Image size:
(3864, 3144)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
S4 Area 2 FIB-SEM Volume 8nm isotropic voxel size, aligned
Files:
Loading...
10. S4 Area 3 FIB-SEM Volume 8nm isotropic
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
4413
Image size:
(5632, 3648)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
S4 Area 3 FIB-SEM Volume 8nm isotropic voxel size, aligned
Files:
Loading...
11. S4 Cell 1 FIB-SEM Volume 5nm isotropic
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
11199
Image size:
(7512, 3864)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(50.0, 50.0)
Details:
S4 Cell 1 FIB-SEM Volume 5nm isotropic voxel size, aligned
Files:
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12. S5 Mock Cell 1 FIB-SEM Volume 8nm isotropic
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
2574
Image size:
(6624, 4236)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
S5 Mock/Control Cell 1 FIB-SEM Volume 8nm isotropic voxel size, aligned
Files:
Loading...
13. Mock cells - Dual axis tilt series
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
26
Image size:
(2024, 2024)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(15.578, 15.578)
Details:
Condition: Mock;
Dual axis tilt series, both axes as individual *.st MRC files
Files:
Loading...
14. Mock cells-Reconstructed tomograms
Category:
reconstructed volumes
Image format:
BIG DATA VIEWER HDF5
No. of images or tilt series:
13
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(15.578, 15.578)
Details:
Condition: Mock;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
Files:
Loading...
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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
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Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)