tilt series

MRC

1

(946, 946)

32 BIT FLOAT

(160.0, 160.0)

Tomogram of reovirus mock-infected U2OS cells (Area2)
EMPIAR entry 10413: U2OS_Reo_MockInfected_Area2_Reconstruction (S-BIAD17)


Note:
Associated with EMPIAR entry 10412 (adjacent/overlapping FOVs)

Sample characteristics:
Substrate = Quantifoil F1 2/2
Fiducials = Au 250nm nanospheres

Data collection:
Data collected at beamline B24 at Diamond Light source
Data collected 18th Feb 2019
Allocation award ID MX21046

Tilt series:
-65deg to 65deg
0.5deg step
Total frames 261
0.5sec exposure

Microscope make:
Zeiss UltraXRM-S220C

Data collection software:
XRM Controller

Microscope setup:
Energy: 500eV
Magnification = x812.5
FOV = 16microns
pixel size = 16x16nm
Zoneplate = 40nm

Data processing:
Type = Automated
Software = Batchruntomo
Alignment protocol = SIRT

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