EMPIAR-10400
Cryo-EM structure of mouse TRPV3 in a nanodisc [1818 multi-frame micrographs composed of 60 frames each in TIFF format]
Publication:

The structure of lipid nanodisc-reconstituted TRPV3 reveals the gating mechanism

Shimada H, Kusakizako T, Dung Nguyen TH, Nishizawa T, Hino T, Tominaga M, Nureki O

Nature structural & molecular biology 27 (2020) 645-652

PMID: 32572254

Related EMDB entry:
Deposited:
2020-04-20
Released:
2020-11-18
Last modified:
2020-11-18
Imageset size:
2.08 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned movies for mouse TRPV3 in a nanodisc
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1818
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.8346, 0.8346)
Details:
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.
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