EMPIAR-10277
Tilt-schemes benchmarking for cryo electron tomography of HIV-1 CA-SP1 [multiple data sets in MRC format]
Publication:

Benchmarking tomographic acquisition schemes for high-resolution structural biology

Turoňová B, Hagen WJH, Obr M, Mosalaganti S, Beugelink JW, Zimmerli CE, Kräusslich HG, Beck M

Nature communications 11 (2020)

PMID: 32054835

Related EMDB entry:
Deposited:
2019-05-17
Released:
2020-03-02
Last modified:
2020-03-02
Imageset size:
48.93 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
1. Unsorted raw tilt-series for benchmarked tilt-schemes
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
35
Image size:
(3708, 3838)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.3269, 1.3269)
Details:
Raw, unsorted, non-dose-filtered tilt-series for 7 different tilt-schemes (5 tilt-series per each tilt-scheme). Each tilt-series contains 41 images - each image is an motion-corrected average of 10-20 frames.
Files:
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2. Tilt-series collected using the dose-symmetric scheme with 3 degrees tilt-step without any gold fiducials
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
8
Image size:
(None, 3838)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.3269, 1.3269)
Details:
Tilt-series acquired using the dose-symmetric tilt-scheme with 3 degrees tilt-step (tilt-range from -60 to +60 degrees) without any gold fiducials. The tilt-series are either not complete (i.e. have less than 41 images) and/or are cut in one dimension to remove any fiducials from the field of view. The trimming was done on the frame level to ensure that the final motion-corrected images do not profit from the presence of any fiducials. The tilt angles are contained in the header. The axis angle for all tilt-series was 85.5 degrees.
Files:
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