EMPIAR-10237
CryoET of mouse protocadherin gamma B6 on membranes (without energy filter) [multiple data sets in MRC format]
Publication:

Visualization of clustered protocadherin neuronal self-recognition complexes

Brasch J, Goodman KM, Noble AJ, Rapp M, Mannepalli S, Bahna F, Dandey VP, Bepler T, Berger B, Maniatis T, Potter CS, Carragher B, Shapiro L

Nature 569 (2019) 280-283

PMID: 30971825

Related EMDB entry:
Deposited:
2018-10-04
Released:
2019-04-18
Last modified:
2022-02-25
Imageset size:
363.41 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • tilt series
1. K2 tilt-series frames
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
61
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.76, 1.76)
Details:
~8 e-/px/s dose rate on the camera
Gain and dark references included
Files:
Loading...
2. Whole-frame aligned tilt images along with all other magnification images from the collection
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
61
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.76, 1.76)
Details:
Whole-frame aligned tilt images using Motioncor2.
Files:
Loading...
3. Appion-Protomo tilt-series alignments
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.76, 1.76)
Details:
Includes a full tilt-series alignment run for two of the tilt-series.
To view properly, navigate to the protomo_alignments folder in Appion-Protomo.
The export/ directory contains SerialEM-formatted unaligned tilt-series stacks, mdoc files, and tilt files. This allows for easy upload to Appion-Protomo.
Files:
Loading...
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)